Liquid Vapor Equilibrium

Exp. 6 Kinetic Characteristics of Acid Phosphatase Introduction Phosphatases argon enzymes that turn the hydrolysis of orthophosphate monoesters with consequent release of inorganic phosphate and are astray distri excepted in nature. The seeds of plants are a particularly enough root word of typical acid phosphatases. The amount of phosphatase body process in seeds normally increases sharply upon germination and then falls as the seedling develops. The hairsplitting physiological function of this phosphatase is not known, but it is probably active in releasing phosphate from organically bound storeho handling forms, such as inositol hexaphosphate (phytic acid), for use in the metabolism of the germinating seedling. In this experiment, acid phosphatase from wheat germ is apply to develop skills in tabing and studying general kinetic properties of enzymes. The assay to be used takes advantage of the broad specificity of the phosphatase b y victimisation the artificial substrate, p-nitro-phenyl phosphate (pNPP). The degree of hydrolysis of the substrate is determined by photometric amount of the p-nitrophenol liberated in the reaction. In alkalescent solution the p-nitrophenolate ion absorbs cast down strongly in the region of 405 nm. Since you are able to use a spectrophotometer and standard cells with a 1.0 cm light cut , you can use the defunctness coefficient for p-nitrophnol in 0.02M NaOH ( ? = 18.8x103 M-1cm-1 at 405 nm) to describe the (moles of product formed from your absorbance readings. Materials and Methods A. Materials: 1. 0.06 M Disodium p-nitrophenyl phosphate (pH 4.8), (pNPP, keep cold) 2. 0.5 M Sodium hydroxide 3. 0.05 U/ml Acid phosphatase in 0.1% bovid serum albumin. 4. 0.025 M Sodium phosphate (pH 4.8) 5. 0.05 M citrate buffer (pH 4.8) B. Equipment: 1. render pipings 2. Pipette and tips 3. Water bath 4. Test tube cru cify 5. Spectrophotometer C. Procedure! : A. Determination of the Michaelis Constant:...If you want to get a full essay, order it on our website: BestEssayCheap.com

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